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| CYT-308 | 重組人巨噬細胞集落刺激因子 （rHuM-CSF） | 2μg/10μg/1mg | Prospec |
| Background: | |
| Macrophage colony stimulating factor （M-CSF） is one of the glycoproteins called colony-stimulating factors （CSFs）.A mononuclear phagocyte colony-stimulating factor （M-CSF） synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo | |
 | Description : |
| Recombinant Human M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD Recombinant M-CSF is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| The lyophilized protein （1mg/ml） was lyophilized with 0.1gr HSA and 0.6gr Manntiol. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution M-CSF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 105 IU/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of M-CSF. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of M-CSF as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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