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CYT-383

重組大鼠白介素-3（rrIL-3）

2μg/10μg/1mg

Prospec

Background :
	Interleukin-3 （IL-3） is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases
	Description :
	Recombinant Rat IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 15783 Dalton. Recombinant Rat IL-3 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Lyophilized from a 1mg/ml solution in water containing 5mM sodium citrate pH-4.
	Solubility:
	It is recommended to reconstitute the lyophilized IL-3 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized Rat IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Interleukin-3 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s rrIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of thymidine uptake by murine MC-9 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 1 x 10⁵ IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Recombinant Rat IL-3.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of Rat IL-3 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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