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BS3, Bis（Sulfosuccinimidyl） Suberate介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

BS3, Bis（Sulfosuccinimidyl） Suberate現貨供應

BS3, Bis（Sulfosuccinimidyl） Suberate品牌介紹

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BS3, Bis（Sulfosuccinimidyl） Suberate僅用于科研

Thermo Scientific Pierce BS3 （Sulfo-DSS） is bis（sulfosuccinimidyl）suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

BS3 contains an amine-reactive N-hydroxysulfosuccinimide （NHS） ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS³ is also available for applications that require a less hydrophilic crosslinker （e.g., to effect intracellular crosslinking）. DSS and BS³ have essentially identical crosslinking activity toward primary amines.

BS³ Highlights

• Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• BS³ is water-soluble, so crosslinking can be done in physiologic solutions
• Water-soluble crosslinking reagent is ideal for cell-surface crosslinking
• High-purity, crystalline BS³ can be used to create high-purity crosslinked conjugates