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DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）現(xiàn)貨供應

DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）品牌介紹

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主要產(chǎn)品包括：

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2）分析試劑

3）熒光標記探針

4）酶聯(lián)免疫分析試劑盒等

DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）僅用于科研

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

3,3´-Dithiobis（sulfosuccinimidylpropionate）  （DTSSP） contains an amine-reactive N-hydroxysulfosuccinimide （sulfo-NHS） ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker. BS³, the non-cleavable analog of DTSSP is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

DTSSP Highlights

• Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
• Cleavable crosslinker allows separation of crosslinked products
• DTSSP crosslinker is not membrane permeable, so cell-surface crosslinking can be done