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重組人粒-巨噬細胞集落刺激因子-sf9(rHuGM-CSF-Sf9)

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CYT-416重組人粒-巨噬細胞集落刺激因子-sf9(rHuGM-CSF-Sf9) 2μg/10μg/1mg Prospec

 

 

Description :
 Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton.
Recombinant Human GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized with no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
N-terminal methionine has been compley removed enzymatically.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec’s Recombinant GM-CSF is fully biologically active when compared to standard.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of
1 x107 IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of GM-CSF .

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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