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產品詳情

CYT-441

重組人骨形態發生蛋白-6（rHuBMP-6）

2μg/10μg/1mg

Prospec

Background :

	Description :
	Recombinant Human BMP-6 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 142 amino acids and having a molecular mass of 15 kDa. 3 additional amino acids were added from the N-terminal Ala-Pro-Thr to increase bacterial expression. BMP-6 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The protein was lyophilized from a concentrated （1mg/ml） sterile solution containing no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized Recombinant BMP-6 in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized Human BMP-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution BMP-6 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
	Amino acid sequence:
	The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser-Ala-Ser-Ser-Arg-Arg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of BMP-6.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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