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| CYT-336 | 重組小鼠血管內(nèi)皮生長因子（rmVEGF） | 2μg/10μg/1mg | Prospec |
| Background: | |
| Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. | |
 | Description : |
| Recombinant Murine VEGF produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 39035 Dalton. Recombinant VEGF is purified by proprietary techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Recombinant VEGF was lyophilized from a concentrated （1mg/ml） solution with no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution murine VEGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |
| Purity: |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Thr. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells （HUVEC） using a concentration range of 1.0-5.0 ng/ml. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of VEGF. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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