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產品詳情

CYT-338

重組人內分泌腺血管內皮生長因子（rHuEG-VEGF）

2μg/10μg/1mg

Prospec

Background:
	Human endocrine gland derived vascular endothelial growth factor （EG-VEGF） is selectively expressed in steroidogenic glands and promotes growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor （SF）-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility.
	Description :
	Recombinant Human EG-VEGF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9605 Dalton. Recombinant EG-VEGF is purified by proprietary techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Recombinant VEGF was lyophilized from a concentrated （1mg/ml） solution with no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuVEGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Ile-Thr-Gly.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells （HUVEC） using a concentration range of 1.0-5.0 ng/ml.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of EG-VEGF.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.