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重組人肌肉生長抑素(rHuMyostatin)
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  • 更新時間:2025-05-02
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CYT-418重組人肌肉生長抑素(rHuMyostatin) 2ug/10ug Prospec

 

 

Description:
 Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass.Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. The myostatin pathway is conserved and regulates muscle mass in a number of animal species ranging from fish to humans. Inhibition of myostatin using a variety of therapeutic approaches can increase muscle mass in a number of animal models of human disease, including muscular dystrophy.
 
Description:
 Recombinant Human Myostatin also called GDF-8 produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 109 amino acids and having a total molecular mass of 24814 Dalton.
Recombinant Human Myostatin is purified by proprietary chromatographic techniques
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 Lyophilized from a concentrated (1mg/ml) solution containing no additives.
 
Solubility:
 It is recommended to reconstitute the lyophilized Myostatin in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized Recombinant Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Myostatin should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be
Asp-Phe-Gly-Leu-Asp.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec’s Recombinant Human Myostatin is fully biologically active when compared to standard. The ED50 as determined by the inhibition of the prolifiration of MPC-11 cells is < 20 ng/ml.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Myostatin.
 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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