PIERCE,Pierce Glycoprotein Staining Kit
- 型號： 24562
- 更新時間： 2025-02-20
- 上一個：24510PIERCE,N-HYDROXYSULFOSUCCINIMIDE（SULFO-NHS）
- 下一個：24590PIERCE,Gelcode Blue Stain Reagent,500 Ml.（see also
訂貨：，，
Pierce Glycoprotein Staining Kit介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
Pierce Glycoprotein Staining Kit現(xiàn)貨供應
| PIERCE | 24562 | Pierce Glycoprotein Staining Kit | KIT |
| PIERCE | 24590 | Gelcode Blue Stain Reagent, 500 Ml. （see also #24592-3.5L） | 500 ML |
| PIERCE | 24596 | GelCode（tm） Blue Safe Protein Stain, 3.5 L. | 3.5 LITER |
Pierce Glycoprotein Staining Kit品牌介紹
美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要產(chǎn)品包括：
1）化學發(fā)光體和比色底物
2）分析試劑
3）熒光標記探針
4）酶聯(lián)免疫分析試劑盒等
Pierce Glycoprotein Staining Kit僅用于科研
The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff （PAS） method.
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins （glycoproteins） that have been separated by polyacrylamide gel electrophoresis （PAGE）. A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff （PAS） reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.
Highlights:
- Specific for glycoproteins – periodic acid-Schiff （PAS） method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
- Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours （half the time required for other staining methods）
- Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
- Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16µg horseradish peroxidase per band
- Controls included – complete kit includes positive and negative control proteins （HRP and STI, respectively）.
- Gels or membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes