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（SMCC） Succinimidyl 4-（N-Maleimidomethyl）Cyclohexane-1-Carbo介紹

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（SMCC） Succinimidyl 4-（N-Maleimidomethyl）Cyclohexane-1-Carbo現(xiàn)貨供應(yīng)

（SMCC） Succinimidyl 4-（N-Maleimidomethyl）Cyclohexane-1-Carbo品牌介紹

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（SMCC） Succinimidyl 4-（N-Maleimidomethyl）Cyclohexane-1-Carbo

僅用于科研

Thermo Scientific Pierce SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm （8.3 angstroms）.

Succinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （SMCC） is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N -hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of SMCC and Sulfo-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.

SMCC Highlights

• Amine reactive NHS ester crosslinks rapidly with primary amine-containing molecule
• Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
• High purity, crystalline SMCC can be used to create high-purity maleimide-activated derivatives
• Cyclohexane bridge confers added stability to the maleimide group making SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C。