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ProFound （tm） Pull-Down GST Protein:Protein Interaction介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

ProFound （tm） Pull-Down GST Protein:Protein Interaction現貨供應

ProFound （tm） Pull-Down GST Protein:Protein Interaction品牌介紹

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ProFound （tm） Pull-Down GST Protein:Protein Interaction僅用于科研

Pierce GST and His Tag Protein Interaction Pull-Down Kits contain the necessary components to capture and purify proteins that interact with GST- or His-tagged fusion proteins, respectively.

You provide the tagged fusion protein as the "bait" and the cells expressing the putative protein interaction target （"prey"）, and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin （agarose beads） and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers. The procedure is conceptually simple.

Highlights:

• GST pull-down （Product No. 21516） – purifies protein interactors of any GST-tagged fusion protein
• 6xHis pull-down （Product No. 21277） – purifies protein interactors of any His-tagged fusion protein
• Complete kits – provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed – use common laboratory equipment and reagents （e.g., microcentrifuge）
• Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible – instructions include protocols for bait and prey proteins from different sources.