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The EZgene^TM Insect gDNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, and some plant tissue samples rich in polysaccharides. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. Samples are homogenized and lysed in a high salt buffer and extracted with chloroform to remove polysaccharides. Following a rapid alcohol precipitation step, binding conditions are adjusted and DNA further purified using ezBind^TM DNA spin columns. In this way, salts, proteins and other contaminants are removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

 

 

All components of the EZgeneTM Insect gDNA Kit, except the Proteinase K and RNase A should be stored at 22^oC-25^oC. Once reconstituted in water, Proteinase K should be stored -20^oC. Under at these conditions, DNA has successfully been purified and used for PCR after 12 months of storage. Store RNase A at 4 ^oC. All EZgene^TM Insect gDNA Kit components are guaranteed for at least 12 months from the date of purchase when stored at 22^oC-25^oC.